Works (35)
Updated: May 15th, 2024 05:23
2024 article
Friedel-Crafts Reactions for Biomolecular Chemistry
Ohata, J. (2024, March 12).
doi.org (repository)
Source: ORCID
Added: March 15, 2024
2024 article
Friedel-Crafts reactions for biomolecular chemistry
Ohata, J. (2024, April 9). ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 4.
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 22, 2024
2024 article
Hexafluoroisopropanol as a Bioconjugation Medium of Ultrafast, Tryptophan-Selective Catalysis
Nuruzzaman, M., Colella, B. M., Uzoewulu, C. P., Meo, A. E., Gross, E. J., Ishizawa, S., … Ohata, J. (2024, February 29). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 2.
Sources: Web Of Science, NC State University Libraries
Added: March 18, 2024
2023 article
Chemical labeling strategies using biomolecule-compatible, nonaqueous media
Ohata, J. (2023, September 27). [Data Management Plan]. DMPTool.].
Source: ORCID
Added: September 28, 2023
2023 journal article
Iron-sensitive protein conjugates formed with a Wittig reaction precursor in ionic liquid
CHEMICAL COMMUNICATIONS, 59(81), 12160–12163.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 25, 2023
2023 article
Iron-sensitive protein conjugates formed with a Wittig reaction precursor in ionic liquid
Nizam, Z. M., Stowe, A. M., Mckinney, J. K., & Ohata, J. (2023, September 15). CHEMICAL COMMUNICATIONS, Vol. 9.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 10, 2023
2023 article
Small-Molecule CO<sub>2</sub> Donors Accelerate Three-Component Peptide/Protein Bioconjugation with Alkylazides and Phosphines in Ionic Liquids
Colella, B. M., & Ohata, J. (2023, December 18). SYNLETT, Vol. 12.
author keywords: multicomponent reaction; CO2 donor; peptide; protein; amine; azide; phosphine
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 2, 2024
2022 article
A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues
Hoshi, K., Messina, M. S., Ohata, J., Chung, C. Y.-S., & Chang, C. J. (2022, May 13). NATURE PROTOCOLS, Vol. 5.
MeSH headings : Animals; Fluorescent Dyes / chemistry; Hydrogen Peroxide / analysis; Mice; Oxidation-Reduction; Signal Transduction; Staining and Labeling
TL;DR:
An optimized two-step, one-pot protocol for synthesizing Peroxymycin-1, an activity-based histochemical probe for hydrogen peroxide detection in fixed cell and mouse tissue samples with improved yields over the originally reported procedure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: May 15, 2022
2022 journal article
Correction to “An Ionic Liquid Medium Enables Development of a Phosphine-Mediated Amine–Azide Bioconjugation Method”
Correction to “An Ionic Liquid Medium Enables Development of a Phosphine-Mediated Amine–Azide Bioconjugation Method.” Journal of the American Chemical Society, 5.
Source: ORCID
Added: May 15, 2022
2022 article
Phosphine-Mediated Three-Component Bioconjugation of Amino- and Azidosaccharides in Ionic Liquids
Ohata, J., Hall, Y., Uzoewulu, C., Nizam, Z., Ishizawa, S., & El-Shaffey, H. (2022, April 18). (Vol. 4). Vol. 4.
Source: ORCID
Added: April 20, 2022
2022 article
Phosphine-mediated three-component bioconjugation of amino- and azidosaccharides in ionic liquids
Hall, Y. D., Uzoewulu, C. P., Nizam, Z. M., Ishizawa, S., El-Shaffey, H. M., & Ohata, J. (2022, August 19). CHEMICAL COMMUNICATIONS, Vol. 8.
MeSH headings : Carbohydrates; Ionic Liquids / chemistry; Phosphines
TL;DR:
This report has developed a bioconjugation strategy for labeling of carbohydrate derivatives through a phosphine-mediated three-component coupling reaction in an ionic liquid medium.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 2, 2022
2022 article
Site-specific DNA functionalization through the tetrazene-forming reaction in ionic liquids
Ishizawa, S., Tumurkhuu, M., Gross, E. J., & Ohata, J. (2022, January 20). CHEMICAL SCIENCE, Vol. 1.
TL;DR:
The non-traditional medium-promoted labeling strategy described here provides an alternative design paradigm for future development of chemical tools for applications involving DNA functionalization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 26, 2022
2022 article
Site-specific DNA functionalization through the tetrazene-forming reaction in ionic liquids (vol 13, pg 1780, 2022)
Ishizawa, S., Tumurkhuu, M., Gross, E. J., & Ohata, J. (2022, June 7). CHEMICAL SCIENCE, Vol. 13, pp. 6749–6751.
TL;DR:
The non-traditional medium-promoted labeling strategy described here provides an alternative design paradigm for future development of chemical tools for applications involving DNA functionalization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 13, 2022
2022 article
Translation of a Phosphine- and Azide-Based Reaction to Chemical Modification of Biomolecules in Ionic Liquid
Uzoewulu, C. P., & Ohata, J. (2022, August 24). SYNLETT, Vol. 7.
author keywords: bioconjugation; ionic liquid; amine; azide; phosphine; phosphazide; iminophosphorane
TL;DR:
This Synpact article describes the pursuit of using an ionic liquid as a nonaqueous reaction medium to conduct phosphine- and azide-based bioconjugation reactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: July 25, 2022
2022 article
Translation of a phosphine- and azide-based reaction to chemical modification of biomolecules in ionic liquid
Uzoewulu, C., & Ohata, J. (2022, July 11).
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: July 11, 2022
2021 journal article
An Ionic Liquid Medium Enables Development of a Phosphine-Mediated Amine-Azide Bioconjugation Method
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 143(33), 12974–12979.
TL;DR:
It is demonstrated that an aprotic ionic liquid serves as a novel reaction solvent for protein bioconjugation without noticeable loss of the biomolecule functions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 14, 2021
2021 journal article
Linear Hydrocarbon Chain Growth from a Molecular Diruthenium Carbide Platform
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 143(39), 16105–16112.
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 16, 2021
2021 article
Site-Specific DNA Functionalization through the Tetrazene-Forming Reaction in Ionic Liquids
Ishizawa, S., Tumurkhuu, M., Gross, E., & Ohata, J. (2021, September 13). (Vol. 9). Vol. 9.
Source: ORCID
Added: September 14, 2021
2021 article
Site-Specific DNA Functionalization through the Tetrazene-Forming Reaction in Ionic Liquids
Ishizawa, S., Tumurkhuu, M., Gross, E., & Ohata, J. (2021, September 13). (Vol. 9). Vol. 9.
Source: ORCID
Added: February 2, 2022
2020 journal article
An Activity-Based Methionine Bioconjugation Approach To Developing Proximity-Activated Imaging Reporters
ACS Central Science, 6(1), 32–40.
TL;DR:
Application of PAIR to activity-based CaM detection provides a method for imaging integrated calcium activity in both in vitro cell and in vivo zebrafish models and can be potentially generalized to target a broader range of analytes by pairing appropriate activity- based protein probes with protein detection reagents in a proximity-driven manner.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2019 journal article
Activity-Based Sensing Methods for Monitoring the Reactive Carbon Species Carbon Monoxide and Formaldehyde in Living Systems
Accounts of Chemical Research.
MeSH headings : Animals; Carbon Monoxide / metabolism; Formaldehyde / metabolism; Humans; Molecular Probe Techniques
TL;DR:
This Account summarizes work from the laboratory on the development of new chemical tools to monitor two important one-carbon RCS, CO and FA, through activity-based sensing (ABS), where the unique chemical reactivities of these small and transient analytes are leveraged, rather than lock-and-key binding considerations, for selective detection.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2019 article
An Activity-Based Methionine Bioconjugation Approach to Developing Proximity-Activated Imaging Reporters
Ohata, J., Krishnamoorthy, L., Gonzalez, M., Xiao, T., Iovan, D., Toste, F. D., … Chang, C. (2019, October 16). (Vol. 10). Vol. 10.
Source: ORCID
Added: October 22, 2021
2019 journal article
Metallvermittelte Funktionalisierung natürlicher Peptide und Proteine: Biokonjugation mit Übergangsmetallen
Angewandte Chemie, 131(19), 6238–6264.
Source: ORCID
Added: December 16, 2020
2019 journal article
Metal‐Mediated Functionalization of Natural Peptides and Proteins: Panning for Bioconjugation Gold
Angewandte Chemie International Edition, 58(19), 6176–6199.
author keywords: bioinorganic chemistry; metalation; peptides; protein modification; transition metals
MeSH headings : Alkylation; Amino Acids / chemistry; Catalysis; Coordination Complexes / chemistry; Gold / chemistry; Metals / chemistry; Oxidation-Reduction; Peptides / chemistry; Peptides / metabolism; Proteins / chemistry; Proteins / metabolism
TL;DR:
Metal-based methods as well as their reactivity and selectivity for the functionalization of natural proteins and peptides for bioconjugation are examined.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2019 journal article
Rapid nickel(ii)-promoted cysteine S-arylation with arylboronic acids
Chemical Communications.
TL;DR:
This work demonstrates an operationally-simple cysteine arylation reaction 2-nitro-substituted arylboronic acids, promoted by a simple nickel(ii) salt, which exhibits strikingly fast reaction rates under physiological conditions in purely aqueous media with excellent selectivity toward Cysteine residues.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2018 journal article
A Naturally Encoded Dipeptide Handle for Bioorthogonal Chan-Lam Coupling
Angewandte Chemie International Edition, 57(15), 4015–4019.
author keywords: bioinorganic chemistry; copper; protein modifications; pyroglutamate; transition metals
TL;DR:
It is reported that pyroglutamate amide N-H bonds exhibit bioorthogonal copper-catalyzed Chan-Lam coupling at pyrogLutamate-histidine dipeptide sequences, allowing specific bioconjugation at a minimalist dipePTide tag.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2018 journal article
A Three-Component Organometallic Tyrosine Bioconjugation
Angewandte Chemie International Edition, 57(11), 2827–2830.
author keywords: arenes; bioinorganic chemistry; protein modifications; rhodium; transition metals
MeSH headings : Animals; Boronic Acids / chemical synthesis; Boronic Acids / chemistry; Catalysis; Cattle; Cell Line; Fluorescent Dyes / chemical synthesis; Fluorescent Dyes / chemistry; Humans; Models, Molecular; Oxidation-Reduction; Peptides / chemical synthesis; Peptides / chemistry; Proteins / chemical synthesis; Proteins / chemistry; Rhodium / chemistry; Salts / chemistry; Tyrosine / chemical synthesis; Tyrosine / chemistry
TL;DR:
An organometallic bioconjugation, employing rhodium(III) salts, to link boronic acids with tyrosine residues by an arene complex is described, allowing incorporation of useful functionalities, such as affinity handles or fluorophores.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2018 journal article
Rhodium at the Chemistry-Biology Interface
Dalton Transactions.
MeSH headings : Animals; Catalysis; Chemistry, Pharmaceutical; DNA / drug effects; Humans; Molecular Structure; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry; Organometallic Compounds / pharmacology; Proteins / antagonists & inhibitors; Proteins / metabolism; Rhodium / chemistry; Rhodium / pharmacology; Structure-Activity Relationship
TL;DR:
A brief review of recent advances in the use of rhodium complexes in biological contexts, including medicinal chemistry, protein science, and chemical biology, highlights both structure- and reactivity-driven approaches to biological probes and discusses how coordination environment affects molecular properties in a biological environment.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2017 journal article
A Hexa-rhodium Metallopeptide Catalyst for Site-Specific Functionalization of Natural Antibodies
Journal of the American Chemical Society, 139(36), 12617–12622.
MeSH headings : Antibodies / chemistry; Catalysis; Humans; Immunoconjugates / chemistry; Metalloproteins / chemistry; Peptides / chemistry; Rhodium / chemistry; Spectrometry, Fluorescence; Tandem Mass Spectrometry
TL;DR:
It is demonstrated that cooperative activity of a multimetallic metallopeptide enables efficient site-specific antibody functionalization, based on molecular recognition of the constant Fc region, which opens a new opportunity for quick and easy production of well-defined antibody conjugates from a variety of antibody sequence and species of origin.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2017 journal article
Ascorbate as a pro-oxidant: mild N-terminal modification with vinylboronic acids
Chemical Communications, 53(10), 1622–1625.
TL;DR:
This mild and selective aqueous reactivity enables selective single-modification of the B chain of human insulin with vinylboronate reagents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: December 16, 2020
2016 journal article
Histidine-Directed Arylation/Alkenylation of Backbone N–H Bonds Mediated by Copper(II)
Journal of the American Chemical Society, 138(24), 7472–7475.
MeSH headings : Alkenes / chemistry; Boronic Acids / chemistry; Catalysis; Copper / chemistry; Histidine / chemistry; Hydrocarbons, Aromatic / chemistry; Hydrogen Bonding; Models, Molecular; Nitrogen / chemistry; Oxidation-Reduction; Thyrotropin-Releasing Hormone / chemistry
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: December 16, 2020
2015 journal article
Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents
Molecular BioSystems, 11(11), 2846–2849.
MeSH headings : Alkynes / chemistry; Animals; Azides / chemistry; Cattle; Click Chemistry / methods; Fluorescent Dyes / chemistry; Fluorescent Dyes / metabolism; Peptides / chemistry; Peptides / metabolism; Protein Processing, Post-Translational; Serum Albumin, Bovine / metabolism
TL;DR:
Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide-alkyne cycloaddition conducted on the surface of a blot membrane is conducted, and sensitivity similar to current commercially available fluorescence imaging methods is possible.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2015 journal article
Luminogenic iridium azide complexes
Chemical Communications, 51(82), 15192–15195.
MeSH headings : Azides / chemical synthesis; Azides / chemistry; Cell Line, Tumor; Coordination Complexes / chemical synthesis; Coordination Complexes / chemistry; Fluorescent Dyes / chemical synthesis; Fluorescent Dyes / chemistry; Humans; Iridium / chemistry; Phenanthrolines / chemical synthesis; Triazoles / chemistry
TL;DR:
The synthesis and characterization of luminogenic, bioorthogonal iridium probes is described, and a simple, modular synthesis via 5-azidophenanthroline allows structural variation and allows optimization of cell labeling.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2015 journal article
Potent and selective inhibition of SH3 domains with dirhodium metalloinhibitors
Chemical Science, 6(8), 4778–4783.
TL;DR:
Specific, designed histidine–rhodium interactions allow a metallopeptide to bind Lyn kinase with nanomolar affinity and to activate kinase activity.
(via Semantic Scholar)
Source: ORCID
Added: December 16, 2020
2014 journal article
A Diruthenium μ-Carbido Complex That Shows Singlet-Carbene-like Reactivity
Journal of the American Chemical Society, 136(45), 15889–15892.
Source: ORCID
Added: December 16, 2020
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