Gregory Kale Buhrman

Works (11)

Updated: April 5th, 2024 03:12

2021 journal article

Structure, Function, and Thermal Adaptation of the Biotin Carboxylase Domain Dimer from Hydrogenobacter thermophilus 2-Oxoglutarate Carboxylase

BIOCHEMISTRY, 60(4), 324–345.

By: G. Buhrman n, P. Enriquez n, L. Dillard n, H. Baer n, V. Truong n, A. Grunden n , R. Rose n 

MeSH headings : Bacteria / enzymology; Bacterial Proteins / chemistry; Carbon-Nitrogen Ligases / chemistry; Crystallography, X-Ray; Enzyme Stability; Hot Temperature; Protein Domains; Structure-Activity Relationship
TL;DR: Structural data reveal that the OGC BC dimer comprises a "wet" dimerization interface that is dominated by hydrophilic interactions and structural water molecules common to all BC domains and likely facilitates the conformational changes associated with the catalytic cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 20, 2021

2015 journal article

Allosteric Effects of the Oncogenic RasQ61L Mutant on Raf-RBD

STRUCTURE, 23(3), 505–516.

By: S. Fetics n, H. Guterres *, B. Kearney n, G. Buhrman n, B. Ma *, R. Nussinov *, C. Mattos n

MeSH headings : Allosteric Regulation; Catalytic Domain; Crystallography, X-Ray; Humans; Hydrogen Bonding; Molecular Dynamics Simulation; Mutation, Missense; Protein Binding; Protein Interaction Domains and Motifs; Protein Structure, Quaternary; Protein Structure, Secondary; Proto-Oncogene Proteins c-raf / chemistry; Proto-Oncogene Proteins p21(ras) / chemistry; Proto-Oncogene Proteins p21(ras) / genetics
TL;DR: In addition to local perturbations on Ras, RasQ61L has substantial long-range effects on the Ras allosteric lobe and on Raf-RBD, which is essential for the activation of the Raf kinase domain through a currently unknown mechanism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Evaluation of a DLA-79 allele associated with multiple immune-mediated diseases in dogs

Immunogenetics, 68(3), 205–217.

By: S. Friedenberg n, G. Buhrman n, L. Chdid n, N. Olby n , T. Olivry n , J. Guillaumin *, T. O’Toole*, R. Goggs * ...

Contributors: S. Friedenberg n, G. Buhrman n, L. Chdid n, N. Olby n , T. Olivry n , J. Guillaumin *, T. O’Toole*, R. Goggs * ...

author keywords: Canine; Immune-mediated disease; Dog leukocyte antigen; Major histocompatibility complex class Ib
MeSH headings : Alleles; Amino Acid Substitution; Animals; Autoimmune Diseases / genetics; Autoimmune Diseases / immunology; Binding Sites; Dog Diseases / genetics; Dog Diseases / immunology; Dogs; Exons; Gene Frequency; Genetic Predisposition to Disease; Genotype; Histocompatibility Antigens Class I / chemistry; Histocompatibility Antigens Class I / genetics; Histocompatibility Antigens Class I / immunology; Immune System Diseases / veterinary; Meta-Analysis as Topic; Models, Molecular; Peptides / chemistry; Peptides / metabolism; Phenotype; Polymorphism, Single Nucleotide; Protein Conformation; Reproducibility of Results; Structure-Activity Relationship
TL;DR: A strong association is demonstrated between two polymorphic positions (10 bp apart) in exon 2 of one allele in D LA-79, DLA-79*001:02, and multiple immune-mediated diseases, resulting in F33L and N37D amino acid changes that are likely to affect critical interactions with the peptide N-terminus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Shift in the Equilibrium between On and Off States of the Allosteric Switch in Ras-GppNHp Affected by Small Molecules and Bulk Solvent Composition

BIOCHEMISTRY, 51(31), 6114–6126.

By: G. Holzapfel n, G. Buhrman n & C. Mattos n

MeSH headings : Acetates / metabolism; Acetates / pharmacology; Allosteric Regulation / drug effects; Allosteric Site / drug effects; Calcium Compounds / metabolism; Calcium Compounds / pharmacology; Catalytic Domain; Dithioerythritol / metabolism; Dithioerythritol / pharmacology; Dithiothreitol / metabolism; Dithiothreitol / pharmacology; Enzyme Stability / drug effects; Guanosine Triphosphate / metabolism; Guanylyl Imidodiphosphate / chemistry; Hydrolysis; Isoenzymes / chemistry; Isoenzymes / metabolism; Models, Molecular; Polyethylene Glycols / pharmacology; Protein Structure, Secondary; Small Molecule Libraries / metabolism; Small Molecule Libraries / pharmacology; Solutions; Solvents / chemistry; Solvents / pharmacology; ras Proteins / chemistry; ras Proteins / metabolism
TL;DR: It is shown with high-resolution structures that calcium acetate and either dithioerythritol (DTE) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence of a moderate amount of poly(ethylene glycol) (PEG) can selectively shift the equilibrium to the "on" state, where the active site appears to be poised for catalysis (calcium acetate), or to what (via Semantic Scholar)
UN Sustainable Development Goal Categories
8. Decent Work and Economic Growth (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Allosteric Modulation of Ras-GTP Is Linked to Signal Transduction through RAF Kinase

JOURNAL OF BIOLOGICAL CHEMISTRY, 286(5), 3323–3331.

By: G. Buhrman*, V. Kumar, M. Cirit n, J. Haugh n  & C. Mattos*

MeSH headings : Allosteric Regulation; Allosteric Site; Animals; Guanosine Triphosphate / metabolism; Kinetics; Mice; Mutation, Missense; NIH 3T3 Cells; Protein Conformation; Protein Stability; Signal Transduction; raf Kinases / metabolism; ras Proteins / genetics; ras Proteins / metabolism
TL;DR: The allosteric switch is linked to the control of signaling in the Ras/Raf/MEK/ERK pathway, supporting a GTPase-activating protein-independent model for duration of the Ras-Raf complex. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2011 journal article

Comparative Analysis of the 15.5kD Box C/D snoRNP Core Protein in the Primitive Eukaryote Giardia lamblia Reveals Unique Structural and Functional Features

BIOCHEMISTRY, 50(14), 2907–2918.

By: S. Biswas n, G. Buhrman n, K. Gagnon *, C. Mattos n, B. Brown  n & E. Maxwell n

MeSH headings : Amino Acid Sequence; Animals; Binding Sites / genetics; Circular Dichroism; Crystallography, X-Ray; Giardia lamblia / metabolism; Humans; Methyltransferases / chemistry; Methyltransferases / metabolism; Models, Molecular; Molecular Sequence Data; Molecular Weight; Phylogeny; Protein Binding; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Protozoan Proteins / chemistry; Protozoan Proteins / genetics; Protozoan Proteins / metabolism; RNA / genetics; RNA / metabolism; Ribonucleoproteins, Small Nucleolar / chemistry; Ribonucleoproteins, Small Nucleolar / classification; Ribonucleoproteins, Small Nucleolar / metabolism; Sequence Homology, Amino Acid; Temperature
TL;DR: Comp comparative sequence analysis indicated a distinct evolutionary position between Archaea and Eukarya, and the Giardia 15.5kD protein occupies a unique position in the evolution of this box C/D RNP core protein retaining structural and functional features characteristic of both archaeal L7Ae and higher eukaryotic 15. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Functional Analysis of a Novel Motif Conserved across Geminivirus Rep Proteins

JOURNAL OF VIROLOGY, 85(3), 1182–1192.

By: T. Nash n, M. Dallas n, M. Reyes n, G. Buhrman n, J. Ascencio-Ibanez  n & L. Hanley-Bowdoin n 

MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Conserved Sequence; DNA Helicases / genetics; DNA Helicases / metabolism; DNA, Viral / metabolism; Geminiviridae / enzymology; Geminiviridae / genetics; Geminiviridae / physiology; Molecular Sequence Data; Mutation, Missense; Plant Diseases / virology; Protein Binding; Sequence Alignment; Tobacco / virology; Trans-Activators / genetics; Trans-Activators / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism; Virulence; Virus Replication
TL;DR: It is established that the GRS is a conserved, essential motif characteristic of an ancient lineage of rolling-circle initiators and support the idea that geminiviruses may have evolved from plasmids associated with phytoplasma or algae. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Allosteric modulation of Ras positions Q61 for a direct role in catalysis

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(11), 4931–4936.

By: G. Buhrman n, G. Holzapfel n, S. Fetics n & C. Mattos n

author keywords: GTPase; Raf; calcium; intrinsic hydrolysis
MeSH headings : Acetates / pharmacology; Allosteric Regulation / drug effects; Allosteric Site; Biocatalysis / drug effects; Calcium / metabolism; Calcium Compounds / pharmacology; Catalytic Domain; Crystallography, X-Ray; Enzyme Stability / drug effects; Glutamine / metabolism; Hydrogen Bonding / drug effects; Hydrolysis / drug effects; Hydroxyl Radical / metabolism; Magnesium / metabolism; Models, Molecular; Protein Structure, Tertiary; Structure-Activity Relationship; ras Proteins / chemistry; ras Proteins / metabolism
TL;DR: This structure suggests a direct catalytic role for Q61 where it interacts with a water molecule that bridges one of the γ-phosphate oxygen atoms to the hydroxyl group of Y32 to stabilize the transition state of the hydrolysis reaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Transformation efficiency of RasQ61 mutants linked to structural features of the switch regions in the presence of Raf

STRUCTURE, 15(12), 1618–1629.

By: G. Buhrman n, G. Wink n & C. Mattos n

MeSH headings : Catalysis; Crystallography, X-Ray; Guanosine Triphosphate / metabolism; Hydrolysis; Models, Molecular; Mutation; Protein Conformation; raf Kinases / metabolism; ras Proteins / chemistry; ras Proteins / genetics
TL;DR: The results suggest that Y32 and a water molecule bridging it to the gamma-phosphate in the wild-type structure play a role in GTP hydrolysis in lieu of the Arg finger in the absence of GAP. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Structural mechanism of oxidative regulation of the phosphatase Cdc25B via an intramolecular disulfide bond

BIOCHEMISTRY, 44(14), 5307–5316.

By: G. Buhrman n, B. Parker n, J. Sohn  n, J. Rudolph  n & C. Mattos n

MeSH headings : Binding Sites; Catalytic Domain; Cell Cycle Proteins / chemistry; Cell Cycle Proteins / metabolism; Crystallization; Disulfides / chemistry; Models, Molecular; Oxidation-Reduction; Protein Conformation; cdc25 Phosphatases / chemistry; cdc25 Phosphatases / metabolism
TL;DR: A crystallographic time course is obtained revealing the structural rearrangements that occur in the P-loop as the enzyme goes from its apo state, through the sulfenic intermediate, to the stable disulfide. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Organic solvents order the dynamic switch II in Ras crystals

STRUCTURE, 11(7), 747–751.

By: G. Buhrman n, V. Serrano n & C. Mattos n

MeSH headings : Crystallization; Guanosine Triphosphate / analogs & derivatives; Guanosine Triphosphate / chemistry; Organic Chemicals / chemistry; Solvents / chemistry; ras Proteins / chemistry
TL;DR: The results suggest that hydrophobic solvents can be used in general to order short biologically relevant segments of disordered regions in protein crystals by favoring H-bonding interactions between atoms that are highly solvated and mobile in aqueous solution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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