| Type of Document |
Master's Thesis |
| Author |
Gantt, John Arthur, |
| Author's Email Address |
jagantt@unity.ncsu.edu |
| URN |
etd-09012003-153904 |
| Title |
Comparative Analysis of RNase P |
| Degree |
Master of Science |
| Graduate Program |
Microbiology |
| Advisory Committee |
| Advisor Name |
Title |
| Dr. James W. Brown |
Committee Chair |
| Dr. Amy M. Grunden |
Committee Member |
| Dr. Stephen J. Libby |
Committee Member |
|
| Keywords |
- holoenzyme
- comparative analysis
- RNase P
|
| Date of Defense |
2003-08-18 |
| Availability |
unrestricted |
Abstract
This Master?s Thesis contains a description of the results of two research projects that were worked on over the course of two years. The first was an attempt to heterologously reconstitute the Bacillus subtilis RNase P RNA with the RNase P protein subunits of Methanothermobacter thermoautotrophicus, to identify a functional homology between one or more of the archaeal proteins with the Bacillus subtilis RNase P protein. Unfortunately, the reconstitution experiment could not be completed due to the poor quality of the pre-tRNAasp substrate synthesized. The second project dealt with comparative analysis of bacterial RNase P RNA. Eleven new RNase P RNA genes were amplified, sequenced, and their secondary structures derived by comparative analysis.
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| Files |
| Filename |
Size |
Approximate Download Time
(Hours:Minutes:Seconds)
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| 28.8 Modem |
56K Modem |
ISDN (64 Kb) |
ISDN (128 Kb) |
Higher-speed Access |
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etd.pdf |
2.39 Mb |
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