Works (1)
Updated: July 5th, 2023 16:02
1999 journal article
Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96(4), 1750–1755.
MeSH headings : Cloning, Molecular; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Herbicides / pharmacokinetics; Inactivation, Metabolic; Insecticides / pharmacokinetics; Kinetics; Microsomes / enzymology; Mixed Function Oxygenases / genetics; Mixed Function Oxygenases / metabolism; Molecular Sequence Data; Phenylurea Compounds / pharmacokinetics; Plants, Genetically Modified; Plants, Toxic; Saccharomyces cerevisiae / enzymology; Saccharomyces cerevisiae / genetics; Soybean Proteins; Soybeans / enzymology; Soybeans / genetics; Substrate Specificity; Tobacco / enzymology; Tobacco / genetics
TL;DR:
In vitro enzyme assays showed that the gene product of one heterologously expressed P450 cDNA (CYP71A10) specifically catalyzed the metabolism of phenylurea herbicides, converting four herbicides of this class into more polar compounds.
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