BIT 510: Core Technologies
Methods and Protocols
Current
Protocols in Molecular Biology
Online and searchable version of Current Protocols in Molecular
Biology. Can also browse by table of contents and view text online.
Search tips: First, click on the title "Current Protocols in
Molecular Biology" in the list of titles. On the next screen,
click on "Search in this title"--located on the upper
right side of the screen. This takes you to a search page for this
specific resource.
Tip: Unit 15.4 in Current
Protocols is titled, "Molecular Cloning of PCR Products."
Some Other Links to Protocols:
Web Resources Protocols page by Cold Spring Harbor Library.
Methods,
Protocols & Laboratory Resources listings from the 123Genomics
web site.
Cloning Tools and Products
Cloning Kits/Procedures
PCR Cloning: Invitrogen
TA Cloning and TOPO TA Cloning:
http://www.invitrogen.com
Search the site for products and technical
information; here is one example of what can be found: PCR
Cloning Considerations.
Commercial Resources for Components:
Enzymes:
http://www.neb.com
Vectors and competent cells:
http://www.stratagene.com
http://www.clontech.com
http://www.novagen.com/ vectors,
vector tables
DNA isolation kits:
http://www.qiagen.com
http://www.promega.com/default.asp
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